The HiTES Platform
Although historically prolific, recent investments into natural products have delivered diminishing returns due to the costly rediscovery of known compounds. This is in part due to the silent nature of most biosynthetic genes: conventional methods only capture ~10% of a bacterium’s biosynthetic potential.
Using a broadly applicable, high-throughput, genetics-free, and cloning-free approach, the Cryptyx platform, HiTES (High-Throughput Elicitor Screening), accesses 90% of this silent biosynthetic potential in a rapid and parallel manner.
Fei Xu, Yihan Wu, Chen Zhang, Katherine M. Davis, Kyuho Moon, Leah B. Bushin, and Mohammad R. Seyedsayamdost
Nature Chemical Biology 15, 161–168 (2019)
Mohammad R. Seyedsayamdost
PNAS 111, 7266–7271 (2014)
Kyuho Moon, Fei Xu, Chen Zhang, Mohammad R Seyedsayamdost
ACS Chemical Biology 14, 767–774 (2019)